Navegando por Autor "Silva, Daniel da"

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Germinação de sementes e estabelecimento in vitro de segmentos nodais de Libidibia Ferrea (Mart. ex Tul.) L. P. Queiroz
(Universidade do Estado do Amazonas, 2023-03-17) Dantas, Alcineia dos Santos; Liberato, Maria Astrid Rocha; Silva, Daniel da; Batista, Ieda Hortencio; Ferreira, Francisca da Silva
The species Libidibia ferrea (Mart. ex Tul.) L.P.Queiroz, popularly known as pau-ferro or jucá, belongs to the Fabaceae family and has great medicinal value due to the potential of its active principle, “pau-ferrol”. This species presents integumentary dormancy with difficulties in the production of seedlings, which impairs its propagation. Thus, the need arises to develop efficient protocols for the propagation of this important medicinal species. This work aimed to establish in vitro propagation methods by testing different substrates, different products for asepsis of explants, as well as the establishment of nodal segments of jucá in vitro. The research was carried out at the Laboratory of Tropical Silviculture and Digital Technologies (LASTED-INPA). Jucá seeds were provided by the Seed Bank of the Microbiology and Soil Fertility Laboratory of the National Institute for Research in the Amazon (INPA). The use of different substrates in the germination of L. ferrea seeds was evaluated, based on the analysis of the germination time period and the percentage of germinated seeds using two types of substrates. In addition, in vitro cultivation of Libidibia ferrea was carried out, using explants taken from germinated seedlings, testing different concentrations of sodium hypochlorite (NaCLO), 70% alcohol, Carbedazin® and Cabrio® Top fungicides. We observed that there were 53% of germinated seeds, testing the substrates organic soil and soil composed of organic with sand in the proportion 1:1, which favored the formation and development of uniform seedlings of L ferrea in ex vitro conditions. The results of the asepsis treatments with different chemical agents did not allow the establishment of a protocol. Keywords: jucá; tissue culture; medicinal plant; micropropagation
Micropropagação de Caesalpinia ferrea Martius
(Universidade do Estado do Amazonas, 2015-04-12) Silva, Daniel da; Sampaio , Paulo de Tarso Barbosa; Rezende , Cleiton Fantin; Albuquerque , Patricia Melchionna; Rapôso, Nádia Verçosa de Medeiros
This project aimed to develop a micropropagation protocol of "Jucá" (Caesalpinia ferrea Mart), an Amazon native species with medicinal, ornamental and timber potential. Seeds were obtained from the Seed Bank belonged to the Laboratoty of Microbiology and Soil Fertility at INPA-Manaus, and after processing, were submerged for 30 minutes in sodium hypochlorite solution of 0.0%, 0.10%, 0.25%, 0.50% and 1.0% and inoculated in MS basal medium. The treatment at 0.25% of sterilizing agent allowed 90% of germination of in vitro seeds. The explants germinated in vitro were targeted and inoculated on MS medium supplemented with 0.1 mg /L of BAP and 0.1 mg /L of IBA. The results indicate that 100% of explants showed shoots and 32.21% developed root systems and total absence of callus. It was observed that 90% of explants derived from yolks developed shoots with 2,73cm and higher proliferation rate (10.21%). The Acclimatization of explants of C. ferrea was possible without the presence of roots grown in vitro, and that the soil is the most suitable substrate for acclimatization with 63.33% of living plants. We conclude that this species can be propagated in vitro, however, further studies are necessary about acclimatization of plants multiplied in vitro in the greenhouse. Keywords: Biotechnology, Vegetal Tissue Culture, in vitro multiplication, Amazon medicinal plant.
Regeneração in vitro de segmentos nodais de Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz sob diferentes concentrações e combinações de reguladores de crescimento e fontes luminosas
(Universidade do Estado do Amazonas, 2019-11-13) Paula, Matheus da Rocha Uchôa de; Liberato, Maria Astrid Rocha; Silva, Daniel da
Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz, popularly known as pau-ferro or jucá, is a plant species belonging to the family Fabaceae-Caesalpinoidae that has great medicinal value due to the potential of its active ingredient "Pau-ferrol A", which can be used as an important tool in the treatment of leukemia Human HL60. However, this species has been undergoing an intense process of extractivism practiced indiscriminately in their natural populations, in addition to of this, causing the genetic erosion and destruction of its habitat in the Amazon, where the irregular seed production combined with high integumentary dormancy has limited the production of seedlings aiming at the recomposition of natural populations and plantations ex situ. The study aimed to evaluate the physiological responses of seedlings of pau-ferro, developed in vitro, under different concentrations and combinations of growth regulators and light sources in the in vitro propagation of ironwood through an efficient alternative to promote the reproduction of genotypes through in vitro conditions of this species. The study was conducted in the National Research Institute's Forestry and Digital Technologies Laboratory da Amazônia (LASTED/INPA) Manaus, Amazonas, where nodal segments, originating from of the in vitro establishment of L. ferrea seeds following Silva's methodology et al., (2018), were inoculated in MS culture medium (SIGMA, M5519-50L, USA) plus different combinations of auxins and cytokinins in concentrations of 0.03 + 0.06 (mgL-1 ) and 0.05 + 0.1 (mgL-1 ), respectively. The auxins used were naphthaleneacetic acid (ANA), indole-3-acetic acid (IAA) and 3-indole-butyric acid (IBA), and the cytokinins were 6-benzylaminopurine (BAP), kinetin (KIN) and thidiazuron (TDZ). The results obtained suggest that the interaction of ANA and BAP (0.05 and 0.1 mg L -1 ), stimulated the highest rate of regeneration under blue-red LED, while the interaction of AIA and BAP (0.05 and 0.1 mg L-1 ) stimulated a higher multiplication rate under White LED, none of the treatments resulted in root growth. Key Words: Cytokinins, auxins, nodal segments, light sources