Práticas metodológicas para colonização de haemagogus janthinomys dyar, 1921 (Diptera: culicidae)

Abstract

Mosquitoes (Diptera: Culicidae) are notable insects of public health importance in the Americas, partly due to their transmission of medically important arboviruses such as dengue, chikungunya and Zika. Some Culicidae of the genus Haemagogus are vectors of sylvatic yellow fever in Brazil. For this reason, studies to improve laboratory rearing and mating techniques are of great importance for certain species as Haemagogus janthinomys and Haemagogus leucocelaenus due the lack of knowledge about vector competence. The present work aims to develop and test several techniques that improve upon existing methods for rearing Haemagogus janthinomys. Adult female mosquitoes were collected at a treefall at the Adolpho Ducke Forest Reserve in north of Manaus, Amazonas, Brazil(2.9633° S, 59.9228° W), between august 2021 and march 2022 using an entomological net and mouth aspirator. Mosquitoes were placed in a cage and provided with water until transferred to an insectary at the Entomology Center of Fundação de Medicina Tropical Doutor Heitor Vieira Dourado to begin rearing activities. These included, blood feeding, oviposition and hatching. The results indicate that 42%, 51% and 7% of females fed, did not feed and died during the feeding process, respectively; the viability and eclosion of 82.8% and 17.5 % of the deposited eggs and development until the adult stage, respectively. The results of oviposition analysis and influence of submersion media did not influence significant statistics, while the survival analysis indicated that females survived longer in control tubes. Key words: mosquitos; Haemagogus; colonização; febre amarela silvestre.