Análise da viabilidade, termoresistência e potencial enzimático de Aspergillus spp. da Central de Coleções Microbiológicas da Universidade do Estado do Amazonas

Abstract

Culture collections are infrastructures that hold organisms or parts of of them, with possible conservation in the short, medium and long term, allowing studies retrospective and prospective, regarding the biology, etiology and virulence aspects of fungi. Objectives: This research aimed to analyze the viability, thermoresistance and enzymatic potential of Aspergillus ssp. deposited at the Microbiological Collections Center from the State University of Amazonas (CCM-UEA). Means and Methods: This is a experimental research developed at CCM-UEA, verifying fungal viability through macro and microscopic characteristics as well as thermal and enzymatic. Results: Of the 18 crops of environmental origin, 100% were viable, having storage for 16, 13 and 12 years. In thermal bioassays at 37ºC, 40ºC and 45ºC, 67% of the strains were positive at 37°C and 56% at 40°C within 72 hours. In Bioassays enzymatic 55% of the strains were positive for proteinases and 11% for phospholipases. Conclusion: Amazonian fungi of the genus Aspergillus, with conservation for periods over 10 years remain viable for laboratory handling, being thermotolerant and enzymatic producers of proteinases and phospholipases, signaling an induction pathway for pathogenesis