Mineração genômica e análise metabolômica de Talaromyces sp., gênero fúngico oriundo do bioma amazônico

Abstract

Microorganisms make up a distinct group of living beings possessing a high metabolic capacity. Among them, the fungal genus Talaromyces stands out because of its ability to biosynthesize compounds of biotechnological interest. To learn about the metabolic pathways related to the production of bioactive compounds, as well as the gene regions responsible for their biosynthesis, this work proposed to investigate the genome of Talaromyces sp., as well as to determine the best cultivation condition for the production of metabolites. In this way, the Talaromyces sp. was submitted to cultivation through the One Strain and Many Active Compounds technique. The extracts obtained were analyzed using Liquid Chromatography coulpled to Mass Spectrometry, and it submitted the results to the Global Natural Products Social Molecular Network platform. It subsequently performed DNA extraction, according to the CTAB protocol. In turn, its genome was sequenced using the Ilumina pared-end 150 bp method. The fungiSmash version 6.1.1 platform, the Geneious software, and the BlastP tool (National Center for Biotechnology Informati) enabled us to carry out gene mining. As metabolomic results, it was possible to identify the best production of metabolites in the Malt Extract medium and dereplicate five molecules of the polyketide class. The mining gene revealed thirty and three biosynthetic gene clusters (BCG’s), being them: 4 ribosomally synthesized and post-translationally modified peptides, 2 terpenes, 13 poliketides, 10 nonribosomal peptides, 1 indole, and two mixed, 1 terpene-polyketide, and 1 nonribosomal peptides-polyketide. At last, through the bibliographic survey, it was possible to relate a cluster to the production of maleic anhydrides, associating it with the biosynthesis of cordianhydrides, in addition to associating homologous enzymes with the synthesis of purpactin A. The metabolome analysis with the mining of the genome of Talaromyces sp. led to the conclusion of the contribution to revealing its biosynthetic potential, in addition to having made possible, through investigations, the elucidation of the biosynthesis of interest compounds. Therefore, it is ratified the need for handling other more refined bioinformatics tools for the study of still-unknown BCG’s, to provide greater precision in the proposals of biosynthetic routes. Keywords: Talaromyces, genome mining, metabolites.