Prevalência dos subtipos virais 16 e 18 de papilomavirus humano (HPV) em mulheres da cidade de Manaus - AM
Carregando...
Arquivos
Data
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade do Estado do Amazonas
Resumo
The natural history of cervical cancer is strongly related to the presence of
papillomavirus (HPV) infection, its relation with cervical cancer is worrisome because
one in each four women has virus contamination. It is estimated that 70% of all
cervical cancers in the world are caused by HPV 16 and 18. Thus, the realization of
this study aimed to contribute to identifying the viral subtypes of circulating HPV in
the population of Manaus-AM. Was then made the collection of cervical sample of 49
women, within the criteria of eligibility of the study, was made DNA extraction from
the samples and analysis of the presence of the virus in the genetic material, by
polymerase chain reaction (PCR), using the generic primers (GP05/06), finally, the
identification of the viral subtypes present, using specific primers of the major
subtypes already studied (16 and 18). Positive HPV-DNA was detected in 100% of
the samples included in the study. HPV 16 was the most prevalent type in most
lesions with 29 (59.2%) of the positive cases and HPV 18 was detected in 4 (8.2%)
women. Multiple infections occurred in 8.2% of cases and infections by other types of
HPV were detected in 40.8% of samples. These data have clinical implications when
considering an effectiveness in the prevention of Cervical Neoplasms of current
prophylactic HPV vaccines, since their coverage, relative to the HPV of oncogenic
high risk, is restricted at 16 and 18.