Avaliação da biodiversidade de bactérias não cultiváveis associadas a anofelinos e seu habitat
| dc.contributor.advisor | Souza , Antonia Queiroz Lima de | |
| dc.contributor.author | Oliveira, Marta Rodrigues de | |
| dc.contributor.co-advisor | Tadei, Wanderli Pedro | |
| dc.contributor.referee | Souza , Antonia Queiroz Lima de | |
| dc.creator.Lattes | http://lattes.cnpq.br/2965070467655985 | pt_BR |
| dc.date.accessioned | 2020-03-17T19:49:54Z | |
| dc.date.accessioned | 2024-09-05T17:29:14Z | |
| dc.date.available | 2020-03-19 | |
| dc.date.available | 2020-03-17T19:49:54Z | |
| dc.date.issued | 2015-04-15 | |
| dc.description.abstract | Malaria is a parasitic disease responsible for millions of deaths annually around the world. Its main vector in Brazil is Anopheles darlingi, Root, 1926. According literature, these vectors are found associated with a great diversity of microorganisms, most of them acquired during immature phase through larvae feeding. Bacteria whose can interfere directly on vetorial capacity of the mosquito highlights these microorganism, and its interaction with immature forms from these vectors in their breeding sites is a relevant factor for stablishing important strategies on transmission control of this disease. Therefore, considering there are no studies about microbial biodiversity associated to A. darlingi larvae and its aquatic habitat, especially on Amazon region, this work has as objective characterize bacterial microbiota associated to these species and its natural and/or artificial breeding sites, in the state of Amazonas-AM. It was collected larvae samples from the water of breeding sites of A. darlingi in the State of Amazonas. DNA extraction from larvae samples was performed through innuPREP Plant DNA® kit, and water samples were submitted to heat shock for cellular lysis. After Genomic DNA extraction, amplification of 16S gene from rDNA was performed, through 27F (5’AGAGTTTGATCMTGGCTCAG-3’) and 1100R (5’-AGGGTTGCGCTCGTT-3’) primers. Amplified products were sequenced, and taxonomic identification was performed by comparison of obtained sequences versus database sequences of 16S rRNA RDP II through Classifier program.16S rRNA region sequencing from 37 samples of 4ºestadio larvae and of water from breeding sites of A. darlingi, generated 827.842 sequences which were grouped into 6.714 bacterial OTUs (Operational Taxonomic Unities). To perform Taxonomical analysis the 20 most significant OTUs were selected, and showed more than 10.000 sequences. From these, it was identified 11 genera, 11 families, 8 orders, 7 classes belonging to Actinobacteria, Bacteroidetes, Firmicutes, Verrucomicrobia e Proteobacteria phyla. Proteobacteria phylum was the most predominant, being present in 80% of analyzed OTUs. Thus, it is shown that A. darlingi and its aquatic habitat hosts a rich bacterial diversity. Keywords: Anopheles darlingi, Biodiversity, Bacterias. | pt_BR |
| dc.description.resumo | A malária é uma doença parasitária responsável por milhares de mortes anualmente em todo o mundo, é transmitida por mosquitos do gênero Anopheles. Tem como principal vetor no Brasil o Anopheles darlingi, Root, 1926. Segundo a literatura estes vetores encontram-se associados a uma grande diversidade de micro-organismos, adquiridos principalmente durante a fase imatura por meio da alimentação larval, dentre os quais se destacam as bactérias, que podem interferir diretamente na capacidade vetorial do mosquito. A interação destas bactérias com as formas imaturas desses vetores em seus criadouros é um fator relevante para estabelecer importantes estratégias de controle da transmissão desta doença. Portanto, considerando que não há estudos sobre a biodiversidade microbiana associada a larvas do A. darlingi e seu habitat aquático, especialmente na Região Amazônica, este trabalho teve como objetivo caracterizar a microbiota bacteriana associada a esta espécie e os seus criadouros naturais e/ou artificiais, no estado do Amazonas-AM. Foram coletadas amostras de larvas e de água dos criadouros de A. darlingi no estado de Amazonas. A extração de DNA das amostras de larvas foi realizada pelo kit innuPREP Plant DNA®, e as amostras de água foram submetidas a choques térmicos para a lise celular. Após a obtenção do DNA genômico, realizou-se a amplificação do gene 16S do rDNA com os primers 27F (5’AGAGTTTGATCMTGGCTCAG-3’) e 1100R (5’-AGGGTTGCGCTCGTT-3’). Os produtos de amplificação obtidos foram sequenciados e a identificação taxonômica foi feita a partir da comparação das sequências obtidas com as sequências depositadas no banco de dados de sequências de 16S rRNA RDP II pelo programa Classifier. O sequenciamento da região 16S rRNA das 37 amostras provenientes de larvas de 4° estádio e de água de criadouros de A. darlingi, gerou 827.842 sequências que foram agrupadas em 6.714 OTUs (Unidades Taxonômicas Operacionais) bacterianas. Para a realização das análises taxonômicas foram selecionadas as 20 OTUs mais representativas, que apresentaram mais de 10.000 sequências. Destas, foram identificados 11 gêneros, 11 famílias, 8 ordens, 7 classes pertencentes aos filos Actinobacteria, Bacteroidetes, Firmicutes, Verrucomicrobia e Proteobacteria. O filo Proteobacteria foi o mais predominante, estando presente em 80% das OTUs analisadas. Desta forma demonstrou-se que as larvas de A. darlingi e seu habitat aquático abrigam uma rica diversidade bacteriana. Palavras-Chave: Anopheles darlingi, Biodiversidade, Bactérias. | pt_BR |
| dc.identifier.uri | https://ri.uea.edu.br/handle/riuea/2063 | |
| dc.language | por | pt_BR |
| dc.publisher | Universidade do Estado do Amazonas | pt_BR |
| dc.publisher.country | Brasil | pt_BR |
| dc.publisher.initials | UEA | pt_BR |
| dc.publisher.program | Programa de Pós-Graduação em Biotecnologia e Recursos Naturais | pt_BR |
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| dc.rights | Acesso Aberto | pt_BR |
| dc.rights | Atribuição-NãoComercial-SemDerivados 3.0 Brasil | * |
| dc.subject | Anopheles darlingi | pt_BR |
| dc.subject | Biodiversidade | pt_BR |
| dc.subject | Bactérias | pt_BR |
| dc.subject.cnpq | Biotecnologia | pt_BR |
| dc.title | Avaliação da biodiversidade de bactérias não cultiváveis associadas a anofelinos e seu habitat | pt_BR |
| dc.type | Dissertação | pt_BR |