Estudo químico e biológico de Casearia javitensis Kunth

Abstract

In Brazil, especially in the Amazon region, there is a great biodiversity of plant species that have not been studied chemical and biologically yet. Therefore the aim of the present study was to perform chemical and biological studies of Casearia javitensis Kunth extracts. Then the hexanic and methanolic extracts as the fractions obtained from the methanolic extract of the branches and leaves from Casearia javitensis have been tested in order to identify the antimicrobial activity by diffusion method well, antioxidant activity (DPPH and Fe3+/phenanthroline) and toxicity against Artemia salina. The fractions of ethyl acetate phase obtained from the methanol extracts of the leaves and branches showed a high antioxidant potential, and the analysis by TLC of these fractions were revealed with FeCl3, indicating the presence of phenolic compounds. The hexanic extract of the branches and leaves showed activity against the bacteria: Corynebacterium glutamicum, Staphylococcus aureus and Bacillus cereus. The methanolic extracts of leaves and branches were active against Pseudomonas aeruginosa, Staphylococcus aureus and Serratia marcescens. The assay against A. salina showed that none of these extracts were considered toxic. The fractionation of the hexane and methanolic extracts of the leaves yielded two compounds: -sitosterol and 4hydroxyphenyl-6-caffeoyl-β-L-glycoside (a phenol-glycoside), respectively, which were identified by 1H and 13C NMR analysis and mass spectrometry.

Keywords: Antioxidant, phenol glycoside, antibacterial, Artemia salina