Análise microbiológica da água do bebedouro da escola estadual de tempo integral Arindal Vinicius da Fonseca Reis, Manicoré-AM

Abstract

Despite being a simple compound made up of two hydrogen and one oxygen (H2O) molecules, water is essential for all life forms on planet Earth. However, obtaining good quality water is becoming increasingly difficult due to the degradation of water sources or even its inadequate management, generated by the increased use of this resource in recent years. Considering the importance of water quality for human health and the several reports of its contamination by Escherichia coli and total coliforms in Brazilian schools, as well as the lack of studies related to its quality evaluation in the city of Manicoré-AM, a study was carried out. An initial study was carried out to determine if the water intended for human consumption offered in public schools in Manicoré complies with the potability standards established in Ordinance no. 2914/2011 from the Ministry of Health. To conduct this study, a drinking fountain from the State School of Integral Time Arindal Vinicius da Fonseca Reis was selected and two microbiological analyzes were performed, with an interval between them for cleaning the internal and external surface from the water fountain. The results of the analyzes revealed the presence of E. coli in 100% of the samples (1st and 2nd microbiological analyzes) and total coliforms in 50% of the samples (1st microbiological analysis), diagnosing that the water offered by the school is unfit for human consumption. In the first microbiological analysis, 1.6 x 103 CFU / 100mL of E. coli and 2.48 x 103 CFU / 100 mL of total coliforms were detected, while the second analysis revealed the presence of 1.6 x 102 CFU / 100 mL E. coli, which characterizes fecal contamination of water. During cleaning procedures, a frog was found on the inner surface of the drinker, so it was possible to establish the hypothesis that fecal contamination is related to this animal, that due to the intense microbial dissemination, only a clean was sufficient to eliminate the entire E. coli population. At the end of this study, the results were passed to the school management and the officials responsible for cleaning the drinking fountain, they also received a lecture and a Standard Operating Protocol (SOP) for the proper cleaning of drinking fountains, which was elaborated for this study. School management and staff are committed to following the passed-on guidelines and taking the necessary steps to ensure water potability. We believe that fecal contamination of drinking water is not related to groundwater contamination and tree frogs are the vectors of E. coli. Keywords: Total Coliforms, Escherichia coli, Fecal Contamination, Importance of cleanliness.