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Análise de degradação do herbicida glifosato por fungos isolados da Bacia do Tarumã Açu
(Universidade do Estado do Amazonas, 2025) Batista, Iago Corrêa; Procópio, Rudi Emerson de Lima; http://lattes.cnpq.br/2478199435796976; http://lattes.cnpq.br/0930544805195803; Batista, Ieda Hortêncio; http://lattes.cnpq.br/5290529604475961; Higa, André Miasato; http://lattes.cnpq.br/4107880506292112; Ferreira, Francisca da Silva; http://lattes.cnpq.br/1723440880004223
Fungi are eukaryotic microorganisms, heterotrophic, and feed by absorption, excreting
enzymes onto the substrate and absorbing the released nutrients. This characteristic makes
them allies of bioremediation, as the metabolites they produce can degrade various substances
depending on the conditions and physiological traits of the microorganism. Bioremediation is
the process of using living organisms to reduce or eliminate pollutants, serving as an
ecologically viable and cost-effective alternative, ideal for the recovery of degraded areas.
Sewage discharges, industrial activities, and the intensive use of pesticides in agriculture are
sources of soil and water contamination. This raises concerns for human health and
biodiversity because these pollutants, often toxic, affect water springs and can cause health
problems and reduce quality of life. Pesticides, such as fungicides, herbicides, and
insecticides, have environmental and health impacts when used indiscriminately. Glyphosate,
the most widely used herbicide in the world, is harmful to human health and the environment,
being genotoxic, cytotoxic, and potentially causing neurological disorders and other health
issues. The Tarumã-Açu Watershed, located in Manaus, is a periurban basin that integrates a
complex water system. Its black, acidic waters, rich in organic compounds, are decomposed
by aquatic microorganisms, forming the base of the local food chain. Although there have
been studies on the recovery of areas affected by pesticides, there is limited research on the
bioremediation and biodegradation of herbicides by aquatic fungi. To examine the
bioremediation potential of fungi isolated from the Tarumã Basin, three fungal isolates
(Aspergillus F2P3, Penicillium F3P2, and Trichoderma F4P3) from the Tarumã-Açu River
Basin were selected. To evaluate the degradation of glyphosate and its possible use as a
source of carbon and phosphorus, the isolates were cultured in media I) Czapek with a low
concentration of sucrose (2.35 g/L), II) medium containing low concentration of sucrose with
the addition of KH2PO4, and III) medium containing low concentration of sucrose with the
addition of glyphosate (2.35 g/L) for 14 days at 28°C with agitation at 150 rpm. The isolates
showed negative results when cultured in the Czapek medium with low sucrose concentration
and supplemented with 2.35 g/L of glyphosate, showing no growth. Chromatography
confirmed the presence of the herbicide, proving its non-degradation. It was concluded that
the analyzed fungi do not have the potential to biodegrade glyphosate.
Keywords: Biodegradation; Herbicide; Environmental impact; Isolation; River Negro
Composição da fauna de abelhas “sem-ferrão” (Hymenoptera: Apidae: Meliponini) registrada no fragmento florestal urbano, Campus I, INPA, Manaus-AM, Brasil
(Universidade do Estado do Amazonas, 2025) Fernandes, Isabel Maia; Reis, Patricia do Rosário; http://lattes.cnpq.br/6276382953849855; http://lattes.cnpq.br/2054299163095342; Carvalho-Zilse, Gislene Almeida; http://lattes.cnpq.br/4767037639322995; Fernandes, Itanna Oliveira; http://lattes.cnpq.br/5126000463150115; Barroso, Paulo Cezar Salgado; http://lattes.cnpq.br/4392663998931242; Cruz, Iris Andrade da; http://lattes.cnpq.br/9033511624061618
This study presents a survey of stingless bee composition (tribe Meliponini) in an urban forest
fragment located at Campus I of the Instituto Nacional de Pesquisas da Amazônia (INPA), in
Manaus-AM, Brazil. Collections were carried out between April and September 2024, using
scent-baited traps and active net sampling. A total of 25 species distributed among 10 genera
were recorded, totaling 1,146 individuals. Based on the diversity indices, we found a moderately
diverse community, with low species dominance and seasonal variations in stingless bee
abundance related to climatic factors such as rainfall. Such diversity in the composition of bees
present in an urban fragment reinforces the importance of these areas as biodiversity refuges and
highlights the relevance of faunal inventories to support conservation and pollinator management
strategies in the Amazon region.
Keywords: Meliponinians. Diversity. Richness. Amazon. Pollinators.
Enzimas hidrolíticas de fungos endofíticos isolados de Eichhornia crassipes (Marth.) Solms de ocorrência no Rio Tarumã-Açu-Amazônia Central
(Universidade do Estado do Amazonas, 2025) Sarges, Raissa Rodrigues; Ferreira, Francisca da Silva; http://lattes.cnpq.br/1723440880004223; http://lattes.cnpq.br/9706205802028539; Liberato, Maria Astrid Rocha; http://lattes.cnpq.br/3718324408807560; Barbosa, Larissa Kirsch; http://lattes.cnpq.br/6547152812685055; Couceiro, Douglas de Moraes; http://lattes.cnpq.br/0014550085642792
Aquatic macrophytes can be colonized by endophytic fungi, microorganisms that inhabit the
internal tissues of plants and play important ecological roles with biotechnological potential,
especially through the production of bioactive metabolites such as extracellular enzymes. The
present study investigated the production of hydrolytic enzymes synthesized by endophytic
fungi that colonize Eichhornia crassipes (Marth.) Solms, which occurs in the lower course of
the Tarumã-Açu River. Aquatic macrophytes were collected in two samples in the lower course
of the Tarumã-Açu River, in Manaus-AM. Isolation was performed at the Biotechnology
Laboratory - ILUM (EST/UEA) and in two stages: antisepsis of leaf and bulb fragments of the
plant; and inoculation of the fragments in 5 equidistant points of Petri dishes containing
PDA+EL medium added with the antibiotics Tetracycline and Ampicillin. Three treatments
were established at different temperatures, with the control treatment at room temperature and
the treatments at 18 °C and 24 °C carried out in B.O.D. incubators at controlled temperature.
Each treatment was performed in triplicate and monitored for 15 days. For the enzymatic tests
of amylase, protease and lipase, solid selective media were used with three-point subtraction
and for the qualitative determination of the enzymatic activities, the Enzymatic Index (IE) was
taken into account. A total of 18 endophytic fungi were isolated, with 7 isolates from the first
collection (Colonization Rate equal to 22%) and 11 from the second collection (Colonization
Rate equal to 36%). The Relative Frequency (Rf) for the leaves was 55.5% while for the bulb
it was 44.4%. Of these isolates, 11 were characterized by their macro and micromorphology,
four were characterized at the genus level because they presented reproductive structures:
Penicillium spp. and Helicosporium sp., two fungi presented reproductive structures to which
it was not possible to define the genus they belonged, and five did not present reproductive
structures, being defined as Mycelia sterilia. Of the 11 endophytic fungi qualitatively analyzed
for the production of extracellular enzymes (amylase, protease and lipase), only the strains of
the genus Penicillium (E.F 1.3.1; E.F. 1.1.1; E.B 2.2.1) resulted positive for protease
production, the fungus Mycelia sterilia (E.B 1.4.1) was positive for amylase, however, none of
the fungi tested presented a positive enzymatic index for lipase. Therefore, these results suggest
that these endophytic fungi isolated from E. crassipes may be promising for hydrolytic enzyme
production activities.
Keywords: Amazon; Endophytic fungi; Enzymes; Aquatic macrophytes
Dança e nutricão: A alimentação como promotora da saúde de bailarinos- intérpretes em períodos pré-apresentação de espetáculos.
(Universidade do Estado do Amazonas, 2024-12-09) Sanatana,Vanessa Giovana Ferreira de; Abreu ,Jeanne Chaves de; http://lattes.cnpq.br/2707740880059445; Abreu ,Jeanne Chaves de; http://lattes.cnpq.br/2707740880059445; Melo ,Cíntia Matos de; http://lattes.cnpq.br/3937764996125913; Silva,Kelson Nunes da; http://lattes.cnpq.br/7889102795322300
This research aims to analyze how dance performers nourish themselves in the periods leading up to a show and the impacts of this nutrition on the body, both positive and negative. The research investigates whether dancers follow specific diets before performances and examines the level of knowledge they have about nutrition, health, and physical performance. Furthermore, it seeks to understand the relationship dancers have with their bodies, how they view nutrition, and how this affects their performance on stage. Through interviews with dance performers, the study reveals that, in many cases, dancers try to follow strict diets, often influenced by aesthetic pressure and the pursuit of an ideal body. However, not everyone has adequate knowledge about the healthiest dietary choices to optimize their physical and emotional performance. Inadequate nutrition, often due to stress and lack of time, can lead to negative effects such as fatigue, a higher risk of injuries, and emotional problems. On the other hand, on stage. Through interviews with dance performers, the study reveals that, in many cases, dancers try to follow strict diets, often influenced by aesthetic pressure and the pursuit of an ideal body. However, not everyone has adequate knowledge about the healthiest dietary choices to optimize their physical and emotional performance. Inadequate nutrition, often due to stress and lack of time, can lead to negative effects such as fatigue, a higher risk of injuries, and emotional problems. On the other hand, used for data collection was the use of field diaries, which allowed for detailed recording of observations and reflections on the dancers' eating habits during the preparation period for the show. The results showed that before the performance, to maintain a body within aesthetic standards, some dancers adopted restrictive diets that occurred abruptly to achieve faster results and the need for a slim body. This research understands that the nutrition of dancer-performers has a direct relationship with the quality of their performance.
Análise da atividade antiviral da glandicolina B em hepatócitos humanos (Huh-7) infectados in vitro pelo vírus dengue
(Universidade do Estado do Amazonas, 2025) Fernandes, Bianca Nadini Teixeira; Lima Júnior, Raimundo Sousa; http://lattes.cnpq.br/2055426262014670; http://lattes.cnpq.br/1631607367333201; Silva, Gladys Corrêa da; http://lattes.cnpq.br/8851211859439121; Ferreira, Rosilene Gomes da Silva; http://lattes.cnpq.br/3376667127204752; Aparício, Cristiane Nazaré Fidelis; http://lattes.cnpq.br/5347208486118179
Dengue is an arboviral disease with a significant impact on global public health, particularly in
tropical and subtropical regions such as Brazil. The high incidence of the disease, combined
with the lack of specific antiviral treatments and the limited efficacy of available vaccines,
underscores the need for new therapeutic strategies. In this context, the present study aimed to
evaluate the antiviral activity of the peptide Glandicolin B, isolated from the fungus Penicillium
rubens, in human hepatocytes (Huh-7) infected in vitro with dengue virus serotype 2 (DENV2). The research was conducted at the Cellular Immunopharmacology Laboratory (LIFCEL) of
the State University of Amazonas. Initially, the cytotoxicity of Glandicolin B was assessed
using the MTT colorimetric assay at various concentrations (0.1 to 100 µg/mL) and exposure
times (24h, 48h, and 72h). The results indicated that the substance did not exhibit significant
toxicity, maintaining cell viability above 90% at all tested concentrations. Subsequently, the
Huh-7 cell line was infected with DENV-2 and treated with Glandicolin B. Antiviral activity
was evaluated by quantifying the viral NS1 protein in the supernatants using the ELISA assay.
The analysis showed a slight reduction in NS1 levels in treated cells, particularly at the
concentration of 100 µg/mL, compared to the untreated infected group; however, the data did
not reach statistical significance. Therefore, although Glandicolin B proved to be safe for use
in Huh-7 cells, it did not significantly inhibit viral replication under the tested conditions. This
result does not invalidate the potential of the compound but suggests the need for further
experiments, including adjustments to incubation time, higher concentrations, coadministration with other compounds, or additional analyses involving other viral markers.
Keyword: Antiviral Molecule; Cytotoxicity; NS1 Antigen; Penicillium.